Course Topics

Day 1

• Origins of Genomic Information
• Sources of Genomic Data
• Introduction to RNA, DNA, and Protein Extraction
• Types of Sequencing
• Nucleotide Sequence Databases
• Primer Design
• BLAST (Basic Local Alignment Search Tool)

Day 2

• Genomic DNA Extraction
• RNA Extraction
• Theoretical and Practical Training in cDNA Synthesis
• Determination of RNA and DNA Concentration and Purity
• Agarose Gel Electrophoresis
• PCR
• Real-Time PCR
• Analysis of Real-Time PCR Results